In which of the following disorders would waxy and broad casts be most likely to be seen?
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Article Microscopy of urine sediment is a simple and inexpensive, but often overlooked procedure. With practice, it can contribute to swift, correct diagnosis of suspected kidney and urinary tract disease. Show
With the development of a wide range of blood tests and multiple test strips (dipsticks) for testing urine, urine microscopy often seems to be forgotten both in everyday use and in the literature on diagnosis of kidney and urinary tract disease. However, existing European guidelines stress the importance of urine examination, including the use of microscopy (1). In the following, a brief summary of these guidelines is illustrated with case histories showing what we may miss by not using a microscope on urine samples. European guidelinesEuropean guidelinesAccording to the guidelines from the European Confederation of Laboratory Medicine (ECLM) (1) there are three important indications for strip testing and microscopy of urine:
The guidelines operate with two levels of knowledge of urine microscopy. The basic level is the minimum for anyone examining urine samples and consists of description and identification of erythrocytes, leukocytes, epithelial cells, hyaline/non-hyaline casts, bacteria, Trichomonas, spermatozoa, artefacts (hairs/fibres etc.), lipid droplets and crystals. At the advanced level, these elements are studied in detail; for example, isomorphic/dysmorphic erythrocytes, subclassification of epithelial cells, casts, bacteria etc. We should point out here that if the strip test is positive, it is important to distinguish between real disease and sample contamination (Fig. 1), which can be determined by microscopy.  Illustrative case historiesPatient 1. The patient was hospitalised with increasing dyspnoea, oedema of the legs, a general sense of malaise and macroscopic haematuria. The patient had a previous medical history of atrial fibrillation, hypertension, ischaemic heart disease and type 2 diabetes. The patient’s clinical status on admission was normal – apart from atrial fibrillation, pitting oedema of the lower extremities and overweight. Blood tests revealed elevated creatinine, 114 μmol/l, with an estimated glomerular filtration rate (eGFR) of 42 ml/min/1.73 m². Urine dipsticks showed abundant leukocytes (> 500 leukocytes/μl urine), a moderate quantity of erythrocytes (≈ 80 erythrocytes/μl) and slight albuminuria (0.3 g/l). Urine microscopy revealed a moderate quantity of isomorphic (intact) erythrocytes (7 – 25 erythrocytes per field of view enlarged 40 ×) (Fig. 2), a moderate quantity of hyaline casts (findings of hyaline casts in every second field of view with an enlargement of 40 ×) and some squamous epithelial cells (1 – 5 squamous epithelial cells per field of view with an enlargement of 40 ×). Further examination with chest X-ray and echocardiogram revealed heart failure, and therapy commenced. A CT abdomen showed localised wall thickening in the urinary bladder, and the patient was referred to a urologist with suspected bladder tumour as the cause of the haematuria. Urine dipsticks react to both intact and damaged erythrocytes and to free haemoglobin and myoglobin, while microscopy may help to determine the source of the erythrocytes in the urine. Isomorphic erythrocytes in the urine mean that the red blood cells are of natural size and shape, in contrast to dysmorphic erythrocyctes, which are deformed after passing through glomerular capillary walls in cases of glomerular disease. They can be detected in the urine as acanthocytes, pencil cells, elliptocytes or fragments. Isomorphic erythrocytes have a regular, biconcave shape, are the result of post-glomerular bleeding and stem from kidney tubules, renal pelvis, ureter, bladder or urethra. Common causes of isomorphic haematuria are pronounced venous congestion in the urinary tract, urinary infections and cancer. These were all possibilities in the patient in question, and a rational test programme was initiated as a result of the urine microscopy. Patient 2. The patient was hospitalised after a clinical history of 24 hours of abdominal pain with intermittent episodes of exacerbation, lethargy and nausea. The clinical examination was normal, apart from hypertension (blood pressure 195/140 mm Hg). In the blood sample, the creatinine value was elevated at 183 μmol/l; the other blood test results were within the normal range. Urine on admission showed the following dipstick levels: leukocytes moderate (≈ 70 – 125 leukocytes/μl), nitrite negative, glucose moderate (14 – 28 mmol/l), albumin moderate (0.3 – 1 g/l), abundant erythrocytes (≈ 200 per μl). Microscopy showed very large numbers of tubular epithelial cells (large flakes) (Fig. 3), a moderate number of cellular casts of tubular epithelium (4 – 10 in the whole slide), moderate numbers of erythrocytes and leukocytes (7 – 25 per field of view with an enlargement of 40 ×) (Fig. 4). The findings were consistent with acute tubular necrosis. X-rays of the abdomen and thorax and a CT without contrast of the urinary tract provided no explanation for the patient’s symptoms. The abdominal pain was interpreted as possible constipation, and the patient received intravenous fluid and antihypertensives. The following day, serum creatinine had risen to 551μmol/l and urine production was falling despite infusion of intravenous fluid. The patient was therefore transferred to the Renal Department. A kidney biopsy showed ischaemic kidney damage (acute tubular necrosis). Three months later the patient was still dependent on dialysis. If the patient’s urine findings had been considered at the time of admission, the renal damage would have been detected before the creatinine value and other blood variables had risen alarmingly. Acute tubular necrosis is acute renal failure where tubular epithelial cells are lost due to ischaemic and/or toxic damage. Microscopy of urine is helpful for distinguishing acute tubular necrosis from prerenal kidney failure, where there is no structural renal damage. In acute tubular necrosis, the urine typically contains tubular epithelial cells and cell casts of tubular epithelium, as in our patient. Patient 3. The patient was hospitalised after some weeks of fatigue and chills, in the last few days with dark urine and pruritus. Blood tests showed a highly elevated creatinine level of 2 449 μmol/l, urea 46.2 mmol/l and potassium 6.4 mmol/l. Urine dipsticks showed considerable proteinuria and haematuria (> 20 g/l and ≈ 200 erythrocytes per μl, respectively). Microscopy showed a nephritic sediment characterised by glomerular haematuria with a moderate quantity of dysmorphic erythrocytes (7 – 25 erythrocytes per field of view with enlargement 40 ×), abundant waxy casts (> 4 in the whole slide). There were also abundant tubular epithelial cells, a moderate presence of granular casts (4 – 10 in the whole slide) and some cellular casts (1 – 3 in the whole slide) (Fig. 5). The patient was transferred the same day to the Renal Department on suspicion of acute glomerulonephritis, which was confirmed by renal biopsy. Appropriate treatment was initiated. Waxy casts are always a serious sign and are only present in patients with pathological conditions. A patient with as high a serum creatinine level as in this case would have been promptly transferred to the Renal Department under any circumstances, but in the present case urine microscopy provided important supplementary information about the urgent need for further diagnostics and treatment. Potential for improvement:Potential for improvement:Although urine microscopy can provide essential and conclusive information in the assessment of a patient’s illness, our impression is that microscopy is often omitted from both literature and practice. For example, a recently published Nordic article on kidney function variables in cases of acute kidney failure did not mention urine microscopy at all (2). Some hospital laboratories do not offer urine microscopy any longer. In our experience, urine microscopy is not as widely used as we think it should be. A study of urine morphology conducted at Diakonhjemmet Hospital in 2008 revealed a great potential for improvement among doctors and biomedical laboratory scientists in satisfying the European guidelines’ minimum requirements for basic knowledge of urine morphology (1, 3). There may be a number of reasons for this. Urinalysis requires a relatively long, thorough training, and the expertise must be maintained. Two Norwegian tools providing support and training in this field were introduced in 2012 (4, 5). Since creatinine, eGFR and cystatin C are widely discussed concerning suspected kidney and urinary disease, it is easy to forget that urine microscopy can provide faster and in many cases more appropriate information. Contaminated samplesContaminated samplesAs mentioned in the introduction, urine microscopy is the only means of determining whether a urine sample is contaminated or not. Urine samples may be contaminated during sampling by vaginal secretions and skin flora if procedures for washing prior to sample collection and mid-stream urine are not properly followed (1, 4, 5). We regard the presence of five or more epithelial cells on microscopy with 40 × enlargement as a sign of contamination. We do not send such a sample for microbiological culture, but report that it is contaminated and recommend new, correct sampling. Contaminated urine samples are a widespread problem (1, 4, 5). We have analysed the samples in our laboratory and found by urine microscopy that approximately 25 % of the over 300 urine samples received each month are contaminated. Cultures of contaminated samples yield results with no clinical relevance and may lead to patients receiving unnecessary antimicrobial treatment if an assumed urinary infection is treated on the basis of a positive strip test without microscopic verification. Culture of contaminated samples will also lead to extra costs for the health service. If the contaminated samples at our hospital had been forwarded for culture at the current rate of NOK 104 per urine sample, it would have resulted in an annual cost of about NOK 100 000 for the hospital. Literature
What are waxy casts in urine?Casts are clusters of urinary sediment elements (red blood cells, white blood cells, fat bodies, etc.) wrapped in a protein matrix. Cast formation necessarily occurs in the kidney tubules, and the contents of the casts indicate the nature of the abnormalities occurring in the kidney itself.
What is casts in urine mean?Fatty casts are seen in people who have lipids in urine. This is most often a complication of nephrotic syndrome . Granular casts are a sign of many types of kidney diseases. Red blood cell casts mean there is a microscopic amount of bleeding from the kidney. They are seen in many kidney diseases.
Which of the following renal disorders frequently occurs following a bacterial infection of the skin or throat?PSGN is a kidney disease that can develop after infections caused by bacteria called group A Streptococcus (group A strep). These infections include: Strep throat.
What does it mean to have hyaline casts in urine?The presence of hyaline casts typically indicates a decreased or sluggish urine flow, which can be a result of strenuous exercise, diuretic medications, severe vomiting, or fever.
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