Formalin-fixed, paraffin embedded tissue block or cell block, or 1 unstained charged slide for each test requested plus 3-4 additional unstained slides cut at 4 microns. Adequate space on each slide for control tissue is required.
Diagnostic Utility
At pH 2.5, Alcian Blue stains sulfated and carboxylated acid mucopolysaccharides and sialomucins [glycoproteins] and is helpful in the diagnosis of Barrett's esophagus and in the identification of mucin secreting adenocarcinomas. The addition of hyaluronidase prior to the stain will further differentiate what types of mucosubstances are present in tissue. Digested and undigested sections are tested in parallel and compared to demonstrate the present of hyaluronic acid, chondroitin 4-sulfate, and chondroitin 6-sulfate in tissue.
Alcian Blue PAS Stain Kit ab245876 is intended for use in the simultaneous histological visualization of sulfated and carboxylated acid mucopolysaccharides, sulfated and carboxylated sialomucins [glycoproteins] and neutral mucins.
Staining Interpretation:
Acidic Sulfated Mucosubstances: Blue
Hyaluronic Acid: Blue
Sialomucins: Blue
Neutral Mucins: Magenta
Mixtures of Acidic and Neutral Mucins: Blue - Mauve depending on dominant entity.
Control Tissue: Small or large intestine, Colon, Appendix.
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
Storage instructions
Store at +4°C. Please refer to protocols.- Components 1 kit Acetic Acid Solution [3%] 2 x 125mlAlcian Blue Solution, pH 2.5 1 x 250mlHematoxylin [Modified Mayer's Solution] 2 x 125mlPeriodic acid solution 1 x 250mlSchiff's solution 1 x 250ml
Images
- Alcian Blue PAS Stain Kit ab245876 Alcian Blue PAS Stain Kit staining formalin-fixed-paraffin embedded human GI tract.
Protocols
Datasheets and documents
SDS download
Datasheet download
References [4]
ab245876 has been referenced in 4 publications.
- Li Y et al. Systematic Characterization of the Disruption of Intestine during Liver Tumor Progression in the xmrk Oncogene Transgenic Zebrafish Model. Cells 11:N/A [2022]. PubMed: 35681505
- Kumar R et al. Gender-based effect of absence of gut microbiota on the protective efficacy of Bifidobacterium longum-fermented rice bran diet against inflammation-associated colon tumorigenesis. Mol Carcinog 61:941-957 [2022]. PubMed: 35856887
- Wietecha MS et al. Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds. Nat Commun 11:2604 [2020]. PubMed: 32451392 Zhang Q et al. Early-stage bilayer tissue-engineered skin substitute formed by adult skin progenitor cells produces an improved skin structure in vivo.
PAS staining is mainly used for staining structures containing a high proportion of carbohydrates such as glycogen, glycoproteins, proteoglycans typically found in connective tissues, mucus and basement membranes.
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38016SS4A
PERIODIC ACID 500ML
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